HPLC Column, NP Amino, Wide Pore, 3um, 2.1mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino™ Wide Pore is a normal-phase and HILIC stationary phase designed for the retention and separation of larger, highly polar compounds. Manufactured using a three-carbon propyl ligand with a terminal amino functional group bonded to ultra-high purity silica, this 300 Å wide pore stationary phase provides enhanced accessibility for larger analytes while maintaining the selectivity associated with USP L8 chemistry. It is an excellent choice as an alternative in USP methods requiring L8 functionality and for method development involving larger polar molecules.
68340-15P-2-3M is an HPLC column packed with 3 µm NP Amino™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 150 mm format. This narrow-bore configuration combines high chromatographic resolution with reduced solvent consumption and compatibility with HILIC-MS applications. The 150 mm length provides increased peak capacity and resolving power compared to shorter column formats, while the wide pore structure improves accessibility for larger analytes. Typical operating flow rates are approximately 0.2–0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of precise and reproducible low-flow operation.
This column is particularly useful for complex sugars, larger compounds containing carboxylic acid groups, and other highly polar analytes analyzed using Normal Phase or HILIC chromatography. The amino functionality provides strong retention for polar molecules, while the 300 Å pore structure accommodates larger analytes that may be restricted on traditional 100 Å materials. As an unmodified silica bonded amino phase, this column is also suitable for HILIC applications. While normal-phase chromatography is generally not the preferred mode for LC-MS, HILIC methods are commonly coupled with mass spectrometry and are well suited to this column format.
Key Benefits
NP Amino™ Wide Pore stationary phase
USP L8 equivalent chemistry
Amino bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for larger molecules
Suitable for Normal Phase chromatography
Suitable for HILIC applications
3 µm particle size
Excellent for HILIC-MS applications
Typical Applications
USP L8 methods
Complex sugar analysis
Carbohydrate analysis
Organic acid analysis
Polar compound analysis
HILIC methods
Normal Phase chromatography
Food and beverage analysis
Pharmaceutical analysis
Biomolecule analysis
Specifications
Product Type: HPLC Column
Stationary Phase: NP Amino™ Wide Pore
USP Classification: L8
Chromatography Modes: Normal Phase and HILIC
Bonded Phase: Amino
Endcapping: Not End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 150 mm
Length: 150 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for larger polar compounds and complex carbohydrates
300 Å wide pores improve accessibility for larger analytes
Suitable for both Normal Phase and HILIC chromatography
As an unmodified silica bonded amino phase, this column is suitable for HILIC applications
The 2.1 mm ID format is ideal for reduced solvent consumption and HILIC-MS compatibility
Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
The 150 mm length provides greater resolution and peak capacity than shorter column formats
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Packed and shipped in Normal Phase solvents
Normal Phase chromatography is not commonly coupled to mass spectrometry, whereas HILIC methods are frequently used with MS detection